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  • Featured Product
  • KD/KO Validated

MON1B Polyclonal antibody

MON1B Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17638-1-AP

Print datasheet

Synonyms

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

验证数据展示

  • WB analysis using 17638-1-AP

    WB analysis using 17638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17638-1-AP (MON1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17638-1-AP

    WB analysis of HeLa using 17638-1-AP

    WB result of MON1B antibody (17638-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected HeLa cells.

  • IF Staining of HeLa using 17638-1-AP

    IF Staining of HeLa using 17638-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (17638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17638-1-AP is MON1B Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen MON1B fusion protein Ag9920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MON1 homolog B (yeast)
Synonyms HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2
Calculated Molecular Weight 547 aa, 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC024277
Gene Symbol MON1B
Gene ID (NCBI) 22879
RRIDAB_2918054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L1V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MON1B antibody 17638-1-APDownload protocol
IF protocol for MON1B antibody 17638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Res

Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.

Authors - Ximing Shao
  • KD Validated
View Article