验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HCT 116 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13842-1-AP targets MOF in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MOF fusion protein Ag4802 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MYST histone acetyltransferase 1 |
| 别名 | MYST1, Lysine acetyltransferase 8, KAT8, hMOF, Histone acetyltransferase KAT8 |
| 计算分子量 | 467 aa, 53 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC037773 |
| 基因名称 | KAT8/MOF |
| Gene ID (NCBI) | 84148 |
| RRID | AB_2146894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H7Z6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994). Western blot analysis detected MOF at an apparent molecular mass of 52 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MOF antibody 13842-1-AP | Download protocol |
| IF protocol for MOF antibody 13842-1-AP | Download protocol |
| IP protocol for MOF antibody 13842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. | ||
Redox Biol Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.
| ||
Mol Cell Proteomics Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). | ||
Mol Cell Proteomics Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human. | ||
Cell Physiol Biochem Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis.
|
