验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31550-1-AP targets MOCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35879 Product name: Recombinant human MOCS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-290 aa of NM_001075098 Sequence: EEGVPLTPKANLLTTEEILTLARLFVKEGIDKIRLTGGEPLIRPDVVDIVAQLQRLEGLRTIGVTTNGINLARLLPQLQKAGLSAINISLDTLVPAKFEFIVRRKGFHKVMEGIHKAIELGYNPVKVNCVVMRGLNEDELLDFAALTEGLPLDVRFIEYMPFDGNKWNFKKMVSYKEMLDTVRQQWPELEKVPEEESSTAK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | molybdenum cofactor synthesis 1 |
| 别名 | KIAA0381, MIG11, MOCOD, MOCS1A, MOCS1B |
| 计算分子量 | 70kDa |
| 观测分子量 | 40-43 kDa |
| GenBank蛋白编号 | NM_001075098 |
| 基因名称 | MOCS1 |
| Gene ID (NCBI) | 4337 |
| RRID | AB_3742448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NZB8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MOCS1 (Molybdenum cofactor synthesis 1), also known as MOCS1A, MOCS1B, MOCOD, plays a crucial role in the biosynthesis of molybdenum cofactor and molybdopterin, which are essential for the activity of several key enzymes involved in carbon, nitrogen, and sulfur metabolism. MOCS1 produces two functional proteins, MOCS1A and MOCS1B, which follow different translocation routes before mitochondrial matrix import for cPMP biosynthesis involving both proteins (PMID: 31996372). MOCS1 was tumor suppressor gene affecting survival positively, while the other genes were oncogenes whose high expression was associated with poor survival (PMID: 37120564). The MOCS1 protein exists multiple isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MOCS1 antibody 31550-1-AP | Download protocol |
| WB protocol for MOCS1 antibody 31550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
