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验证数据展示

  • WB analysis of K-562 using 15886-1-AP

    WB analysis of K-562 using 15886-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15886-1-AP (MOBKL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15886-1-AP

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15886-1-AP

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of K-562 using 15886-1-AP

    IP experiment of K-562 using 15886-1-AP

    IP result of anti-MOBKL3 (IP:15886-1-AP, 4ug; Detection:15886-1-AP 1:300) with K-562 cells lysate 4000ug.

MOBKL3 Polyclonal antibody

MOBKL3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15886-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15886-1-AP targets MOBKL3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8671

Product name: Recombinant human MOBKL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC005237

Sequence: MVMAEGTAVLRRNRPGTKAQDFYNWPDESFDEMDSTLAVQQYIQQNIRADCSNIDKILEPPEGQDEGVWKYEHLRQFCLELNGLAVKLQSECHPDTCTQMTATEQWIFLCAAHKTPKECPAIDYTRHTLDGAACLLNSNKYFPSRVSIKESSVAKLGSVCRRIYRIFSHAYFHHRQIFDEYENETFLCHRFTKFVMKYNLMSKDNLIVPILEEEVQNSVSGESEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MOB1, Mps One Binder kinase activator-like 3 (yeast)
别名 PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D
计算分子量 225 aa, 26 kDa
观测分子量 24 kDa
GenBank蛋白编号BC005237
基因名称 MOBKL3
Gene ID (NCBI) 25843
RRIDAB_10858478
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MOBKL3 antibody 15886-1-APDownload protocol
IHC protocol for MOBKL3 antibody 15886-1-APDownload protocol
IP protocol for MOBKL3 antibody 15886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols