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验证数据展示

  • WB analysis using 15325-1-AP

    WB analysis using 15325-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15325-1-AP (MOAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15325-1-AP

    IHC staining of mouse brain using 15325-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15325-1-AP (MOAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MOAP1 antibody included
  • IHC staining of mouse brain using 15325-1-AP

    IHC staining of mouse brain using 15325-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15325-1-AP (MOAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MOAP1 antibody included

MOAP1 Polyclonal antibody

MOAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15325-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MAP 1, MAP1, MOAP1, modulator of apoptosis 1, Paraneoplastic antigen Ma4, PNMA4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MOAP1 Ready-To-Use IHC Kit. MOAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15325-1-AP targets MOAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag2876

Product name: Recombinant human MOAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC015044

Sequence: MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 modulator of apoptosis 1
别名 MAP 1, MAP1, MOAP1, modulator of apoptosis 1, Paraneoplastic antigen Ma4, PNMA4
计算分子量 39 kDa
观测分子量36-39 kDa
GenBank蛋白编号BC015044
基因名称 MOAP1
Gene ID (NCBI) 64112
RRIDAB_3085459
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Modulator of apoptosis 1 (MOAP1, also known as MAP-1 and PNMA4) belongs to the PNMA family and is expressed in various types of tissues (PMID: 36724555). MAP1 contains a central BH3-like motif and interacts with the BAX protein (PMID: 11060313). It plays a central role in mitochondria and death receptor-mediated apoptosis and is associated with many diseases such as cancers and neurological diseases (PMID: 35269511).

实验方案

Product Specific Protocols
WB protocol for MOAP1 antibody 15325-1-APDownload protocol
IHC protocol for MOAP1 antibody 15325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Res Sq

The Retrotransposon-Derived Capsid Genes PNMA1 and PNMA4 Maintain Reproductive Capacity

Authors - Luke Berchowitz
View Article
mouseWB

bioRxiv

The retrotransposon - derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity

Authors - Thomas W P Wood
  • KO Validated
View Article