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MOAP1 Polyclonal antibody

MOAP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15325-1-AP

Print datasheet

Synonyms

MAP 1, MAP1, MOAP1, modulator of apoptosis 1, Paraneoplastic antigen Ma4, PNMA4

验证数据展示

  • WB analysis using 15325-1-AP

    WB analysis using 15325-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15325-1-AP (MOAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15325-1-AP

    IHC staining of mouse brain using 15325-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15325-1-AP (MOAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MOAP1 antibody included
  • IHC staining of mouse brain using 15325-1-AP

    IHC staining of mouse brain using 15325-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15325-1-AP (MOAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MOAP1 antibody included

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MOAP1 Ready-To-Use IHC Kit. MOAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15325-1-AP targets MOAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MOAP1 fusion protein Ag2876 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name modulator of apoptosis 1
Synonyms MAP 1, MAP1, MOAP1, modulator of apoptosis 1, Paraneoplastic antigen Ma4, PNMA4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight36-39 kDa
GenBank Accession NumberBC015044
Gene Symbol MOAP1
Gene ID (NCBI) 64112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BY2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Modulator of apoptosis 1 (MOAP1, also known as MAP-1 and PNMA4) belongs to the PNMA family and is expressed in various types of tissues (PMID: 36724555). MAP1 contains a central BH3-like motif and interacts with the BAX protein (PMID: 11060313). It plays a central role in mitochondria and death receptor-mediated apoptosis and is associated with many diseases such as cancers and neurological diseases (PMID: 35269511).

实验方案

Product Specific Protocols
WB protocol for MOAP1 antibody 15325-1-APDownload protocol
IHC protocol for MOAP1 antibody 15325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols