验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
15325-1-AP targets MOAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2876 Product name: Recombinant human MOAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC015044 Sequence: MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | modulator of apoptosis 1 |
| 别名 | MAP 1, MAP1, MOAP1, modulator of apoptosis 1, Paraneoplastic antigen Ma4, PNMA4 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 36-39 kDa |
| GenBank蛋白编号 | BC015044 |
| 基因名称 | MOAP1 |
| Gene ID (NCBI) | 64112 |
| RRID | AB_3085459 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BY2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Modulator of apoptosis 1 (MOAP1, also known as MAP-1 and PNMA4) belongs to the PNMA family and is expressed in various types of tissues (PMID: 36724555). MAP1 contains a central BH3-like motif and interacts with the BAX protein (PMID: 11060313). It plays a central role in mitochondria and death receptor-mediated apoptosis and is associated with many diseases such as cancers and neurological diseases (PMID: 35269511).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MOAP1 antibody 15325-1-AP | Download protocol |
| WB protocol for MOAP1 antibody 15325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Res Sq The Retrotransposon-Derived Capsid Genes PNMA1 and PNMA4 Maintain Reproductive Capacity | ||
bioRxiv The retrotransposon - derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity
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