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验证数据展示

  • WB analysis of rat testis using 12693-1-AP

    WB analysis of rat testis using 12693-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12693-1-AP (MNS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12693-1-AP

    IHC staining of human testis using 12693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12693-1-AP (MNS1 Antibody) at dilution of 1:50 (under 40x lens).

MNS1 Polyclonal antibody

MNS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12693-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

meiosis nuclear structural 1, MNS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12693-1-AP targets MNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MNS1 fusion protein Ag3423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 meiosis-specific nuclear structural 1
别名 meiosis nuclear structural 1, MNS1
计算分子量 495 aa, 61 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC034991
基因名称 MNS1
Gene ID (NCBI) 55329
RRIDAB_2877875
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEH6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Meiosis-specific nuclear structural 1 (MNS1), is abundantly expressed in post-meiotic spermatids and is required for proper flagellar formation and function. Male mice deficient in MNS1 display decreased sperm production and are sterile because their sperm are immotile. The microtubules and ODFs of the flagella are disrupted, resulting in abnormal, short sperm tails. In addition, MNS1 is also required for motile ciliary functions.

实验方案

Product Specific Protocols
WB protocol for MNS1 antibody 12693-1-APDownload protocol
IHC protocol for MNS1 antibody 12693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols