×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HeLa using 10136-1-AP (same clone as 10136-1-PBS)

    WB analysis of HeLa using 10136-1-AP (same clone as 10136-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 10136-1-AP (same clone as 10136-1-PBS)

    IHC staining of mouse pancreas using 10136-1-AP (same clone as 10136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10136-1-AP (MNK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 10136-1-AP (same clone as 10136-1-PBS)

    IHC staining of mouse pancreas using 10136-1-AP (same clone as 10136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10136-1-AP (MNK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10136-1-AP (same clone as 10136-1-PBS)

    IF Staining of HepG2 using 10136-1-AP (same clone as 10136-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MNK1 antibody (10136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

MNK1 Polyclonal antibody, PBS Only

MNK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10136-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MKNK1, EC:2.7.11.1, MAP kinase signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAPK signal-integrating kinase 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

10136-1-PBS targets MNK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0184

Product name: Recombinant human MKNK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 299-465 aa of BC002755

Sequence: PPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAP kinase interacting serine/threonine kinase 1
别名 MKNK1, EC:2.7.11.1, MAP kinase signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAPK signal-integrating kinase 1
计算分子量 51 kDa
观测分子量 51 kDa, 47 kDa
GenBank蛋白编号BC002755
基因名称 MNK1
Gene ID (NCBI) 8569
RRIDAB_2250527
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUB5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MNK1 (mitogen-activated protein kinase-interacting kinase 1) was discovered as a consequence of bacterial expression libraries screening for proteins regulated by the classical mitogen-activated protein kinases, the ERKs (extracellular signal-regulated kinases). Phosphorylation of Mnk1 has been shown to activate its kinase activity as well as to enhance its binding to the eukaryotic initiation factor 4G (eIF4G) which functions as a scaffolding protein. Human MNK1 arises from an alternatively spliced transcript giving rise to 2 isoforms, longer MNK1a (50 kDa) and a shorter MNK1b variant (38 kDa) that lacks 89 C-terminal amino acids(PMID: 21406405).

||
New chat

Able™

正在加载，请稍候...