验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, MCF-7 cells, NIH/3T3 cells, A431 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | A375 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MNAT1 Ready-To-Use IHC Kit. MNAT1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
11719-1-AP targets MNAT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2293 Product name: Recombinant human MNAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC000820 Sequence: MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEALEVERQENEQRRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFSTGIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGGYTSSLACHRALQDAFSGLFWQPS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) |
| 别名 | Cyclin-G1-interacting protein, Cyclin G1 interacting protein, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, CDK7/cyclin H assembly factor |
| 计算分子量 | 309 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC000820 |
| 基因名称 | MNAT1 |
| Gene ID (NCBI) | 4331 |
| RRID | AB_2145054 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51948 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MNAT1 antibody 11719-1-AP | Download protocol |
| IP protocol for MNAT1 antibody 11719-1-AP | Download protocol |
| WB protocol for MNAT1 antibody 11719-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Therapeutic Targeting of CDK7 Suppresses Tumor Progression in Intrahepatic Cholangiocarcinoma. | ||
BMC Cancer MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway.
| ||
Acta Histochem Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice. | ||
Cell Rift Valley fever virus coordinates the assembly of a programmable E3 ligase to promote viral replication | ||
