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验证数据展示

  • WB analysis using 86551-3-RR (same clone as 86551-3-PBS)

    WB analysis using 86551-3-RR (same clone as 86551-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86551-3-RR (MMS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86551-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 86551-3-RR (same clone as 86551-3-PBS)

    IHC staining of human colon cancer using 86551-3-RR (same clone as 86551-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86551-3-RR (MMS2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86551-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMS2 antibody included
  • IHC staining of human colon cancer using 86551-3-RR (same clone as 86551-3-PBS)

    IHC staining of human colon cancer using 86551-3-RR (same clone as 86551-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86551-3-RR (MMS2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86551-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMS2 antibody included

MMS2 Recombinant monoclonal antibody, PBS Only

MMS2 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86551-3-PBS

产品说明书

CloneNo. 251485F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

UBE2V2, DDVit 1, DDVIT1, EDAF 1, EDAF-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MMS2 Ready-To-Use IHC Kit. MMS2 primary antibody included.

产品信息

86551-3-PBS targets MMS2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1111

Product name: Recombinant human UBE2V2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-145 aa of BC007051

Sequence: MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2 variant 2
别名 UBE2V2, DDVit 1, DDVIT1, EDAF 1, EDAF-1
计算分子量 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC007051
基因名称 MMS2
Gene ID (NCBI) 7336
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15819
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMS2 encodes a 137-amino acid, 15.2-kDa protein with significant sequence homology to a conserved family of ubiquitin-conjugating (Ubc) proteins (PMID: 9576943). Mms2 is a ubiquitin E2-variant protein with a very well-documented function in the tolerance pathway that protects both human and yeast cells from the lethal and mutagenic effects of DNA damage (PMID: 36934633). A high expression level of human MMS2 is associated with poor survival prognosis in different cancer diseases (PMID: 36934633).

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