验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 17 publications below |
| CoIP | See 1 publications below |
产品信息
16015-1-AP targets MMS19 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MMS19 fusion protein Ag8792 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MMS19 nucleotide excision repair homolog (S. cerevisiae) |
| 别名 | hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L |
| 计算分子量 | 1030 aa, 113 kDa |
| 观测分子量 | 103-113 kDa |
| GenBank蛋白编号 | BC006575 |
| 基因名称 | MMS19 |
| Gene ID (NCBI) | 64210 |
| RRID | AB_2145043 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96T76 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MMS19 antibody 16015-1-AP | Download protocol |
| IF protocol for MMS19 antibody 16015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.
| ||
Cell Metab Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.
| ||
Nat Commun Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases. | ||
Cell Rep The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.
| ||
Mol Metab Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. |