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MMP8 Recombinant antibody

MMP8 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230030G11

Cat No : 82885-1-RR

Print datasheet

Synonyms

CLG1, HNC, Matrix metalloproteinase 8, MMP 8, MMP8, Neutrophil collagenase, PMNL CL, PMNL collagenase

验证数据展示

  • WB analysis using 82885-1-RR

    WB analysis using 82885-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82885-1-RR (MMP8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHSC-T6 cells, K-562 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82885-1-RR targets MMP8 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name matrix metallopeptidase 8 (neutrophil collagenase)
Synonyms CLG1, HNC, Matrix metalloproteinase 8, MMP 8, MMP8, Neutrophil collagenase, PMNL CL, PMNL collagenase
Calculated Molecular Weight 467 aa, 53 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC074988
Gene Symbol MMP8
Gene ID (NCBI) 4317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22894
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa,respectively(PMID: 28772283, PMID:15042023).

实验方案

Product Specific Protocols
WB protocol for MMP8 antibody 82885-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols