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验证数据展示

  • WB analysis using 30655-1-AP (same clone as 30655-1-PBS)

    WB analysis using 30655-1-AP (same clone as 30655-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30655-1-AP (MMP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 30655-1-AP (same clone as 30655-1-PBS)

    IHC staining of mouse spleen using 30655-1-AP (same clone as 30655-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30655-1-AP (MMP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 30655-1-AP (same clone as 30655-1-PBS)

    IF Staining of A549 using 30655-1-AP (same clone as 30655-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MMP25 antibody (30655-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

MMP25 Polyclonal antibody, PBS Only

MMP25 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30655-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MTMMP6, MT6MMP, MT MMP6, MMP 25, matrix metallopeptidase 25

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30655-1-PBS targets MMP25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29304

Product name: Recombinant human MMP25 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 260-381 aa of NM_022468

Sequence: GDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 25
别名 MTMMP6, MT6MMP, MT MMP6, MMP 25, matrix metallopeptidase 25
计算分子量 63 kDa
观测分子量38-40 kDa
GenBank蛋白编号NM_022468
基因名称 MMP25
Gene ID (NCBI) 64386
RRIDAB_3669745
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPA2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP25, also known as MT6-MMP, is one of the two glycosylphosphatidylinositol anchored matrix metalloproteinases (MMPs) that have been suggested to play a role in pericellular proteolysis. MMP25 was originally cloned from human leukocytes and its mRNA is predominantly expressed in leukocytes (polymorphonuclear cells and monocytes) of normal tissues and in brain, colon, urothelial and prostate cancers (PMID: 17513868). MMP-25 can exaggerate inflammatory responses by inactivating the main antiproteinase, a1-proteinase inhibitor or serpin (PMID: 16584348). The band at 57-kDa we detected is likely to be active MT6-MMP and minor MT6-MMP degradation product of 38-kDa.

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