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MMP23B Polyclonal antibody

MMP23B Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13020-1-AP

Print datasheet

Synonyms

Femalysin, matrix metallopeptidase 23B, Matrix metalloproteinase 21, Matrix metalloproteinase 22, Matrix metalloproteinase 23, MIFR, MIFR 1, MMP 21, MMP 22, MMP 23, MMP21, MMP22, MMP23A, MMP23B

验证数据展示

  • WB analysis of A549 using 13020-1-AP

    WB analysis of A549 using 13020-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 13020-1-AP

    IHC staining of human ovary tumor using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 13020-1-AP

    IHC staining of human ovary tumor using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 13020-1-AP

    IF Staining of HepG2 using 13020-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13020-1-AP (MMP23B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 13020-1-AP

    FC experiment of HepG2 using 13020-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug MMP23B antibody (13020-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

13020-1-AP targets MMP23B in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MMP23B fusion protein Ag4113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 23B
Synonyms Femalysin, matrix metallopeptidase 23B, Matrix metalloproteinase 21, Matrix metalloproteinase 22, Matrix metalloproteinase 23, MIFR, MIFR 1, MMP 21, MMP 22, MMP 23, MMP21, MMP22, MMP23A, MMP23B
Calculated Molecular Weight 390 aa, 44 kDa
Observed Molecular Weight 46-60 kDa
GenBank Accession NumberBC025719
Gene Symbol MMP23B
Gene ID (NCBI) 8510
RRIDAB_2146152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP23B(matrix metallopeptidase 23B) is also named as MMP21, MMP22.It is a regulator in matrix remodeling and may be playing a role in enchondral bone formation and in regulation of cranial suture closure .It has 3 isoforms produced by alternative splicing with the molecular weight of 44KDa,48KDa and 46KDa,respectively.It also can be N-glycosylated.

实验方案

Product Specific Protocols
WB protocol for MMP23B antibody 13020-1-APDownload protocol
IHC protocol for MMP23B antibody 13020-1-APDownload protocol
IF protocol for MMP23B antibody 13020-1-APDownload protocol
FC protocol for MMP23B antibody 13020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
View Article