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验证数据展示

  • WB analysis of A549 using 13020-1-AP

    WB analysis of A549 using 13020-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13020-1-AP

    IF Staining of HepG2 using 13020-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP23B antibody (13020-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 13020-1-AP

    FC experiment of HepG2 using 13020-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MMP23B Polyclonal antibody (13020-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human ovary tumor using 13020-1-AP

    IHC staining of human ovary tumor using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 13020-1-AP

    IHC staining of human ovary tumor using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MMP23B Polyclonal antibody

MMP23B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 13020-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

EC:3.4.24.-, Femalysin, matrix metallopeptidase 23B, Matrix metalloproteinase 21, Matrix metalloproteinase 22

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13020-1-AP targets MMP23B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag4113

Product name: Recombinant human MMP23B protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 52-390 aa of BC025719

Sequence: QGDVAALGLSAVPPTRVPGPLAPRRRRYTLTPARLRWDHFNLTYRILSFPRNLLSPRETRRALAAAFRMWSDVSPFSFREVAPEQPSDLRIGFYPINHTDCLVSALHHCFDGPTGELAHAFFPPHGGIHFDDSEYWVLGPTRYSWKKGVWLTDLVHVAAHEIGHALGLMHSQHGRALMHLNATLRGWKALSQDELWGLHRLYGCLDRLFVCASWARRGFCDARRRLMKRLCPSSCDFCYEFPFPTVATTPPPPRTKTRLVPEGRNVTFRCGQKILHKKGKVYWYKDQEPLEFSYPGYLALGEAHLSIIANAVNEGTYTCVVRRQQRVLTTYSWRVRVRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 23B
别名 EC:3.4.24.-, Femalysin, matrix metallopeptidase 23B, Matrix metalloproteinase 21, Matrix metalloproteinase 22
计算分子量 390 aa, 44 kDa
观测分子量 46-60 kDa
GenBank蛋白编号BC025719
基因名称 MMP23B
Gene ID (NCBI) 8510
RRIDAB_2146152
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP23B(matrix metallopeptidase 23B) is also named as MMP21, MMP22.It is a regulator in matrix remodeling and may be playing a role in enchondral bone formation and in regulation of cranial suture closure .It has 3 isoforms produced by alternative splicing with the molecular weight of 44KDa,48KDa and 46KDa,respectively.It also can be N-glycosylated.

实验方案

Product Specific Protocols
IF protocol for MMP23B antibody 13020-1-APDownload protocol
IHC protocol for MMP23B antibody 13020-1-APDownload protocol
WB protocol for MMP23B antibody 13020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
View Article
humanWB,IHC

Curr Eye Res

Cohesin Complex Interacting with Promoters of MMP Genes for in Pterygium Occurrence

Authors - Shichao Han
View Article
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