验证数据展示

WB analysis using 10373-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10373-2-AP (MMP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of U-87 MG using 10373-2-AP
WB result of MMP2 antibody (10373-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-87 MG cells.
IP experiment of MCF-7 using 10373-2-AP
IP result of anti-MMP2 (IP:10373-2-AP, 4ug; Detection:10373-2-AP 1:300) with MCF-7 cells lysate 2400 ug.
IHC staining of human gliomas using 10373-2-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with MMP2 antibody included
IHC staining of human gliomas using 10373-2-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10373-2-AP (MMP2 Antibody) at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with MMP2 antibody included
IF Staining of human placenta using 10373-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using MMP2 antibody (10373-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10373-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP2 antibody (10373-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

MMP2 Polyclonal antibody

MMP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10373-2-AP

产品说明书

引用文献(1212)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (7)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, EC:3.4.24.24, Gelatinase A

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (1212)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HT-1080 cells, MCF-7 cells, U-87 MG cells, HepG2 cells, U-251 cells, J774A.1 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human gliomas tissue, human placenta tissue, human prostate cancer tissue, human heart tissue, human oesophagus tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue
Positive IF/ICC detected in	HepG2 cells

In sample preparation, the extracellular matrix needs to be included

Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 1034 publications below
IHC	See 161 publications below
IF	See 65 publications below
IP	See 1 publications below

产品信息

10373-2-AP targets MMP2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse
文献引用反应性	human, mouse, pig, rabbit, monkey, chicken, bovine, goat, fish
免疫原	CatNo: Ag0549 Product name: Recombinant human MMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 461-660 aa of BC002576 Sequence: LGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
别名	72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, EC:3.4.24.24, Gelatinase A
计算分子量	72 kDa
观测分子量	62-72 kDa
GenBank蛋白编号	BC002576
基因名称	MMP2
Gene ID (NCBI)	4313
RRID	AB_2250823
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P08253
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).

实验方案

Product Specific Protocols
IF protocol for MMP2 antibody 10373-2-AP	Download protocol
IHC protocol for MMP2 antibody 10373-2-AP	Download protocol
IP protocol for MMP2 antibody 10373-2-AP	Download protocol
WB protocol for MMP2 antibody 10373-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	J Hematol Oncol Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway. Authors - Qing Xie View Article
human	WB	Mol Cancer Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study. Authors - Erfei Chen View Article
human	WB	Mol Cancer Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer. Authors - Jianan Chen View Article
human	WB	Mol Cancer Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals. Authors - Dingwei Xue View Article
human	WB	Mol Cancer Down-regulation of miR-675-5p contributes to tumor progression and development by targeting pro-tumorigenic GPR55 in non-small cell lung cancer. Authors - Dan He View Article
human	WB	Mol Cancer MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-α. Authors - Shuo Chen View Article

验证数据展示

WB analysis using 10373-2-AP

WB analysis of U-87 MG using 10373-2-AP

IP experiment of MCF-7 using 10373-2-AP

IHC staining of human gliomas using 10373-2-AP

IHC staining of human gliomas using 10373-2-AP

IF Staining of human placenta using 10373-2-AP

IF Staining of HepG2 using 10373-2-AP

MMP2 Polyclonal antibody

MMP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

In sample preparation, the extracellular matrix needs to be included

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway.

Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.

Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer.

Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals.

Down-regulation of miR-675-5p contributes to tumor progression and development by targeting pro-tumorigenic GPR55 in non-small cell lung cancer.

MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-α.

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