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验证数据展示

  • WB analysis using 31166-1-AP

    WB analysis using 31166-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31166-1-AP (MMGT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 31166-1-AP

    IHC staining of mouse kidney using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of human colon cancer using 31166-1-AP

    IHC staining of human colon cancer using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of human colon cancer using 31166-1-AP

    IHC staining of human colon cancer using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of mouse adrenal gland using 31166-1-AP

    IHC staining of mouse adrenal gland using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of mouse colon using 31166-1-AP

    IHC staining of mouse colon using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of rat adrenal gland using 31166-1-AP

    IHC staining of rat adrenal gland using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of rat adrenal gland using 31166-1-AP

    IHC staining of rat adrenal gland using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of rat colon using 31166-1-AP

    IHC staining of rat colon using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included
  • IHC staining of rat kidney using 31166-1-AP

    IHC staining of rat kidney using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMGT1 antibody included

MMGT1 Polyclonal antibody

MMGT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31166-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TMEM32, Transmembrane protein 32

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-468 cells, PC-3 cells, SW480 cells, U-251 cells
Positive IHC detected inrat adrenal gland tissue, mouse kidney tissue, human colon cancer tissue, mouse adrenal gland tissue, mouse colon tissue, rat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® MMGT1 Ready-To-Use IHC Kit. MMGT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31166-1-AP targets MMGT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35121

Product name: Recombinant human MMGT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 65-131 aa of BC033588

Sequence: GEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVLFRPSDTANSSNQDALSSNTSLKLRKLESLRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane magnesium transporter 1
别名 TMEM32, Transmembrane protein 32
计算分子量 131 aa, 15 kDa
观测分子量15-20 kDa
GenBank蛋白编号BC033588
基因名称 MMGT1
Gene ID (NCBI) 93380
RRIDAB_3742401
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8N4V1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMGT1 (Membrane Magnesium Transporter 1) is a protein encoded by the MMGT1 gene in humans. It is also known by the aliases EMC5 and TMEM32. This protein is a member of the magnesium transporter family and is involved in the transport of magnesium ions across cell membranes.

实验方案

Product Specific Protocols
IHC protocol for MMGT1 antibody 31166-1-APDownload protocol
WB protocol for MMGT1 antibody 31166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

EMBO J

Principles of ribosome-associated protein quality control during the synthesis of CFTR.

Authors - Tom Joshua Oldfield
View Article
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