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验证数据展示

  • WB analysis of HEK-293 using 60034-3-Ig

    WB analysis of HEK-293 using 60034-3-Ig

    WB result of Neprilysin/CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neprilysin/CD10 transfected HEK-293 cells.

  • WB analysis using 60034-3-Ig

    WB analysis using 60034-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60034-3-Ig

    WB analysis using 60034-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 60034-3-Ig

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human kidney using 60034-3-Ig

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human liver using 60034-3-Ig

    IHC staining of human liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human liver using 60034-3-Ig

    IHC staining of human liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • Featured Product
  • KD/KO验证

Neprilysin/CD10 Monoclonal antibody

Neprilysin/CD10 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60034-3-Ig

产品说明书

CloneNo. 4B9D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig, rabbit

应用

WB, IHC, ELISA

别名

MME, Neprilysin, 4B9D9, Atriopeptidase, CALLA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, Daudi cells, HEK-293 cells, mouse kidney tissue, pig kidney tissue, Ramos cells, A172 cells, rabbit kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human renal cell carcinoma tissue, human stomach cancer tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:25000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60034-3-Ig targets Neprilysin/CD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, pig, rabbit
免疫原

CatNo: Ag0427

Product name: Recombinant human MME,CD10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 52-252 aa of BC101658

Sequence: YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 membrane metallo-endopeptidase
别名 MME, Neprilysin, 4B9D9, Atriopeptidase, CALLA
计算分子量 750 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC101658
基因名称 CD10
Gene ID (NCBI) 4311
RRIDAB_10641991
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08473
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

实验方案

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 60034-3-IgDownload protocol
IHC protocol for Neprilysin/CD10 antibody 60034-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols