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验证数据展示

  • WB analysis of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    WB analysis of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    IF Staining of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MMACHC antibody (66609-1-Ig, Clone: 4G10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66609-1-Ig (same clone as 66609-1-PBS)

    FC experiment of HeLa using 66609-1-Ig (same clone as 66609-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human MMACHC (66609-1-Ig, Clone:4G10A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

MMACHC Monoclonal antibody, PBS Only

MMACHC Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66609-1-PBS

产品说明书

CloneNo. 4G10A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

4G10A11, Alkylcobalamin:glutathione S-alkyltransferase, CblC, cyanide-eliminating, Cyanocobalamin reductase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66609-1-PBS targets MMACHC in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 MMACHC fusion protein Ag15001 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
别名 4G10A11, Alkylcobalamin:glutathione S-alkyltransferase, CblC, cyanide-eliminating, Cyanocobalamin reductase
计算分子量 282 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006122
基因名称 MMACHC
Gene ID (NCBI) 25974
RRIDAB_2881969
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y4U1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.