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MMACHC Polyclonal antibody

MMACHC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21462-1-AP

Print datasheet

Synonyms

cblC, MMACHC, RP11 291L19.3

验证数据展示

  • IHC staining of human liver using 21462-1-AP

    IHC staining of human liver using 21462-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 21462-1-AP

    IHC staining of human liver using 21462-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Raji using 21462-1-AP

    WB analysis of Raji using 21462-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21462-1-AP (MMACHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21462-1-AP is MMACHC Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MMACHC fusion protein Ag15001 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Synonyms cblC, MMACHC, RP11 291L19.3
Calculated Molecular Weight 282 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC006122
Gene Symbol MMACHC
Gene ID (NCBI) 25974
RRIDAB_10732959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4U1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.

实验方案

Product Specific Protocols
WB protocol for MMACHC antibody 21462-1-APDownload protocol
IHC protocol for MMACHC antibody 21462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols