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验证数据展示

  • WB analysis of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

    WB analysis of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

    IP experiment of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

    IP result of anti-MMAB (IP:11137-1-AP, 4ug; Detection:11137-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

    IF Staining of HeLa using 11137-1-AP (same clone as 11137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MMAB antibody (11137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 11137-1-AP (same clone as 11137-1-PBS)

    IHC staining of human ovarian cancer using 11137-1-AP (same clone as 11137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 11137-1-AP (MMAB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11137-1-PBS in a different storage buffer formulation.

MMAB Polyclonal antibody, PBS Only

MMAB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11137-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ATP:co(I)rrinoid adenosyltransferase MMAB, ATR, cblB, Corrinoid adenosyltransferase MMAB, EC:2.5.1.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11137-1-PBS targets MMAB in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1602

Product name: Recombinant human MMAB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-250 aa of BC011831

Sequence: MAVCGLGSRLGLGSRLGLRGCFGAARLLYPRFQSRGPQGVEDGDRPQPSSKTPRIPKIYTKTGDKGFSSTFTGERRPKDDQVFEAVGTTDELSSAIGFALELVTEKGHTFAEELQKIQCTLQDVGSALATPCSSAREAHLKYTTFKAGPILELEQWIDKYTSQLPPLTAFILPSGGKISSALHFCRAVCRRAERRVVPLVQMGETDANVAKFLNRLSDYLFTLARYAAMKEGNQEKIYMKNDPSAESEGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methylmalonic aciduria (cobalamin deficiency) cblB type
别名 ATP:co(I)rrinoid adenosyltransferase MMAB, ATR, cblB, Corrinoid adenosyltransferase MMAB, EC:2.5.1.-
计算分子量 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC011831
基因名称 MMAB
Gene ID (NCBI) 326625
RRIDAB_2235498
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EY8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

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