验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, A431 cells, human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
55102-1-AP targets AF-6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 |
| 别名 | AF 6, AF6, AFADIN, Afadin variant, MLLT4 |
| 计算分子量 | 207 kDa |
| 观测分子量 | 182-210 kDa |
| GenBank蛋白编号 | NM_005936 |
| 基因名称 | Afadin/AF6 |
| Gene ID (NCBI) | 4301 |
| RRID | AB_10792809 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55196 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MLLT4, also named as AF6 and Afadin, belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It is a nNectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. The antibody is specific to MLLT4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AF-6 antibody 55102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice | ||
Res Sq Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance | ||
Aging (Albany NY) The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level |
