验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HL-60 cells, Raji cells, mouse retina tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy KMT2E/MLL5 Ready-To-Use IHC Kit. KMT2E/MLL5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
14540-1-AP targets MLL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6044 Product name: Recombinant human MLL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC062583 Sequence: MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVGIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRNLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQHISKCKKAFREGSRKSSRVKGSAPEIDPSSDGSNFGWETKIKAWMDRYEEANNNQYSEGVQREAQRIALRLGNGNDKKEMNKSDLNTNNLLFKPPVESHIQKNKKILKSAKDLPPDAL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) |
| 别名 | NKp44L, Myeloid/lymphoid or mixed-lineage leukemia protein 5, KMT2E, Inactive lysine N-methyltransferase 2E, Inactive histone-lysine N-methyltransferase 2E |
| 计算分子量 | 205 kDa |
| 观测分子量 | 100 kDa, 70 kDa |
| GenBank蛋白编号 | BC062583 |
| 基因名称 | MLL5 |
| Gene ID (NCBI) | 55904 |
| RRID | AB_2878065 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IZD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MLL5 antibody 14540-1-AP | Download protocol |
| IHC protocol for MLL5 antibody 14540-1-AP | Download protocol |
| WB protocol for MLL5 antibody 14540-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
