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- KD/KO Validated
MLKL Polyclonal antibody
MLKL Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, HepG2 cells, mMSCs cells |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21066-1-AP targets MLKL in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, rat, pig |
Immunogen | MLKL fusion protein Ag15188 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mixed lineage kinase domain-like |
Synonyms | BC028141, MLKL |
Calculated Molecular Weight | 471 aa, 54 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC028141 |
Gene Symbol | MLKL |
Gene ID (NCBI) | 197259 |
RRID | AB_2878804 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8NB16 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MLKL antibody 21066-1-AP | Download protocol |
IHC protocol for MLKL antibody 21066-1-AP | Download protocol |
IF protocol for MLKL antibody 21066-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Host Microbe Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.
| ||
Front Immunol N-acetyl-L-cysteine alleviated the oxidative stress-induced inflammation and necroptosis caused by excessive NiCl2 in primary spleen lymphocytes | ||
Cell Death Discov MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury | ||
Cancers (Basel) STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma. | ||
Am J Chin Med Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis. | ||
Front Cell Dev Biol Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model. |