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MLF2 Polyclonal antibody

MLF2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11835-1-AP

Print datasheet

Synonyms

MLF2, myeloid leukemia factor 2, NTN4

验证数据展示

  • WB analysis of human brain using 11835-1-AP

    WB analysis of human brain using 11835-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11835-1-AP (MLF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11835-1-AP

    IHC staining of human gliomas using 11835-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11835-1-AP (MLF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11835-1-AP

    IHC staining of human gliomas using 11835-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11835-1-AP (MLF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11835-1-AP

    IF Staining of HeLa using 11835-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11835-1-AP (MLF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11835-1-AP targets MLF2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen MLF2 fusion protein Ag2270 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myeloid leukemia factor 2
Synonyms MLF2, myeloid leukemia factor 2, NTN4
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC000898
Gene Symbol MLF2
Gene ID (NCBI) 8079
RRIDAB_2235413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15773
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLF2 antibody 11835-1-APDownload protocol
IHC protocol for MLF2 antibody 11835-1-APDownload protocol
IF protocol for MLF2 antibody 11835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
View Article
humanIF

Adv Sci (Weinh)

MLF2 Negatively Regulates P53 and Promotes Colorectal Carcinogenesis

Authors - Debao Fang
View Article