验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27012-1-AP targets MKX in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MKX fusion protein Ag25549 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mohawk homeobox |
| 别名 | C10orf48, Homeobox protein Mohawk, IFRX, IRXL1, mohawk homeobox |
| 计算分子量 | 352 aa, 39 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC036207 |
| 基因名称 | MKX |
| Gene ID (NCBI) | 283078 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYA7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mohawk (Mkx) is a member of the Three Amino Acid Loop Extension (TALE) superclass of atypical homeobox genes. It encodes a transcriptional repressor that is expressed in the embryonic precursors of skeletal muscle, tendon, cartilage, and bone. This gene is highly conserved across species and is known to be involved in the differentiation and maintenance of these tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MKX antibody 27012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |