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验证数据展示

  • IF Staining of HepG2 using CL488-66349

    IF Staining of HepG2 using CL488-66349

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66349 (MKP-2 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MKP-2 Monoclonal antibody

MKP-2 Monoclonal Antibody for IF
Cat No. CL488-66349

产品说明书

CloneNo. 1G1C12

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rat, mouse

应用

IF

别名

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66349 targets MKP-2 in IF applications and shows reactivity with human, pig, rat, mouse samples.

经测试应用 IF Application Description
经测试反应性 human, pig, rat, mouse
免疫原

CatNo: Ag1206

Product name: Recombinant human MKP-2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-394 aa of BC014565

Sequence: MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dual specificity phosphatase 4
别名 dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2
计算分子量 43 kDa
观测分子量 43 kDa, 33 kDa
GenBank蛋白编号BC014565
基因名称 DUSP4
Gene ID (NCBI) 1846
RRIDAB_2883315
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13115
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MKP-2 antibody CL488-66349Download protocol
Standard Protocols
Click here to view our Standard Protocols