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CoraLite® Plus 488-conjugated MKP-2 Monoclonal antibody

MKP-2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1C12

Cat No : CL488-66349

Print datasheet

Synonyms

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2

验证数据展示

  • IF Staining of HepG2 using CL488-66349

    IF Staining of HepG2 using CL488-66349

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66349 (MKP-2 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66349 targets MKP-2 in IF applications and shows reactivity with human, pig, rat, mouse samples.

Tested Applications IF/ICC
Tested Reactivity human, pig, rat, mouse
Immunogen MKP-2 fusion protein Ag1206 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dual specificity phosphatase 4
Synonyms dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa, 33 kDa
GenBank Accession NumberBC014565
Gene Symbol DUSP4
Gene ID (NCBI) 1846
RRIDAB_2883315
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13115
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MKP-2 antibody CL488-66349Download protocol
Standard Protocols
Click here to view our Standard Protocols