验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, RAW 264.7 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66349-1-Ig targets MKP-2 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, pig, rat, mouse samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC, CoIP |
| 经测试反应性 | human, pig, rat, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1206 Product name: Recombinant human MKP-2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-394 aa of BC014565 Sequence: MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase 4 |
| 别名 | dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa, 33 kDa |
| GenBank蛋白编号 | BC014565 |
| 基因名称 | DUSP4 |
| Gene ID (NCBI) | 1846 |
| RRID | AB_2881729 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13115 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MKP-2 antibody 66349-1-Ig | Download protocol |
| IHC protocol for MKP-2 antibody 66349-1-Ig | Download protocol |
| WB protocol for MKP-2 antibody 66349-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration | ||
Cell Rep DUSP4 promotes esophageal squamous cell carcinoma progression by dephosphorylating HSP90β
| ||
Cancers (Basel) Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases | ||
Life Sci Alliance DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathway |
