验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, rat pancreas tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 6 publications below |
产品信息
22772-1-AP targets MIXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag18732 Product name: Recombinant human MIXL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 147-232 aa of BC113441 Sequence: KSFQPLARPEIILNHCAPGTETKCLKPQLPLEVDVNCLPEPNGVGGGISDSSSQGQNFETCSPLSEDIGSKLDSWEEHIFSAFGNF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Mix1 homeobox-like 1 (Xenopus laevis) |
| 别名 | MIX1 homeobox-like protein 1, Mix.1 homeobox-like protein, MIX, MILD1, Homeobox protein MIXL1 |
| 计算分子量 | 232 aa, 25 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC113441 |
| 基因名称 | MIXL1 |
| Gene ID (NCBI) | 83881 |
| RRID | AB_2879164 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9H2W2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mix1 homeobox-like 1 (MIXL1) Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. This antibody is specific to MIXL1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MIXL1 antibody 22772-1-AP | Download protocol |
| WB protocol for MIXL1 antibody 22772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. | ||
Cell Death Differ Uric acid: a potent molecular contributor to pluripotent stem cell cardiac differentiation via mesoderm specification. | ||
Front Cell Dev Biol Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells | ||
STAR Protoc Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells. |
