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验证数据展示

  • WB analysis using 88330-1-RR (same clone as 88330-1-PBS)

    WB analysis using 88330-1-RR (same clone as 88330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88330-1-RR (MIRO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88330-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 88330-1-RR (same clone as 88330-1-PBS)

    IHC staining of human colon using 88330-1-RR (same clone as 88330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 88330-1-RR (MIRO2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88330-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 88330-1-RR (same clone as 88330-1-PBS)

    IF Staining of HepG2 using 88330-1-RR (same clone as 88330-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MIRO2 antibody (88330-1-RR, Clone: 260828B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 88330-1-PBS in a different storage buffer formulation.

MIRO2 Recombinant monoclonal antibody, PBS Only

MIRO2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 88330-1-PBS

产品说明书

CloneNo. 260828B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RHOT2, ARHT2, C16orf39, EC:3.6.5.-, hMiro 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88330-1-PBS targets MIRO2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1752

Product name: Recombinant human RHOT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 319-618 aa of BC014942

Sequence: DRDGALSPVELQSLFSVFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ras homolog gene family, member T2
别名 RHOT2, ARHT2, C16orf39, EC:3.6.5.-, hMiro 2
计算分子量 68 kDa
观测分子量 68-75 kDa
GenBank蛋白编号BC014942
基因名称 RHOT2
Gene ID (NCBI) 89941
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IXI1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle. MIRO2 is highly phosphorylated at basal conditions, as evidenced by an immunoreactive band that electrophoretically migrates 7 kDa higher (~75 kDa) compared to its predicted molecular weight (68 kDa) (PMID: 28556983).

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