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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83856-5-RR (same clone as 83856-5-PBS)

    WB analysis using 83856-5-RR (same clone as 83856-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83856-5-RR (same clone as 83856-5-PBS)

    WB analysis of HeLa using 83856-5-RR (same clone as 83856-5-PBS)

    WB result of MIOS antibody (83856-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIOS transfected HeLa cells. This data was developed using the same antibody clone with 83856-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83856-5-RR

    Affinity and Kinetic Characterization of 83856-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-5-RR against Human MIOS were performed. The affinity constant is below 1 pM.

MIOS Recombinant monoclonal antibody, PBS Only

MIOS Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 83856-5-PBS

产品说明书

CloneNo. 240832B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

240832B9, GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83856-5-PBS targets MIOS in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15148

Product name: Recombinant human MIOS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 188-537 aa of BC005883

Sequence: AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAVPHQGRGFSQYGVSGSPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 missing oocyte, meiosis regulator, homolog (Drosophila)
别名 240832B9, GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog
计算分子量 875 aa, 99 kDa
观测分子量 82 kDa
GenBank蛋白编号BC005883
基因名称 MIOS
Gene ID (NCBI) 54468
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NXC5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510