验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13137-1-AP targets MINK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3795 Product name: Recombinant human MINK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-304 aa of BC034673 Sequence: MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | misshapen-like kinase 1 (zebrafish) |
| 别名 | B55, EC:2.7.11.1, GCK family kinase MiNK, hMINK, hMINKbeta |
| 计算分子量 | 1332 aa, 150 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC034673 |
| 基因名称 | MINK1 |
| Gene ID (NCBI) | 50488 |
| RRID | AB_10644151 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N4C8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Misshapen/Nck-interacting kinase (NIK)-related kinase 1 (MINK1), also known as mitogen-activated protein 4 kinase 6, a serine-threonine protein kinase belonging to the sterile 20 kinase superfamily, has been implicated in diverse physiological and pathophysiological processes, including platelet activation and thrombus formation (PMID: 38723371). MINK1 has the potential to modulate the activation of NLRP3 inflammasome, indicating its pivotal role in governing pyroptosis. Overall, MINK1 is known to regulate cell senescence, cell motility and migration (PMID: 36182968). MINK1 expression has been demonstrated in multiple tissues and organs, including nervous tissue, kidneys, cancer tissues, and immune system cells. (PMID: 39727954)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MINK1 antibody 13137-1-AP | Download protocol |
| IP protocol for MINK1 antibody 13137-1-AP | Download protocol |
| WB protocol for MINK1 antibody 13137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway | ||
Cardiovasc Ther Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats. | ||
Front Genet MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma | ||
Front Immunol Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling |
