MINK1 Polyclonal antibody
MINK1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13137-1-AP targets MINK1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB,ELISA |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MINK1 fusion protein Ag3795 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | misshapen-like kinase 1 (zebrafish) |
| Synonyms | B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3 |
| Calculated Molecular Weight | 1332 aa, 150 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC034673 |
| Gene Symbol | MINK1 |
| Gene ID (NCBI) | 50488 |
| RRID | AB_10644151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4C8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MINK1 antibody 13137-1-AP | Download protocol |
| IHC protocol for MINK1 antibody 13137-1-AP | Download protocol |
| IP protocol for MINK1 antibody 13137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway | ||
Cardiovasc Ther Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats. | ||
Front Genet MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma |
