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验证数据展示

  • WB analysis using 86760-3-RR (same clone as 86760-3-PBS)

    WB analysis using 86760-3-RR (same clone as 86760-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86760-3-RR (MINA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86760-3-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 86760-3-RR (same clone as 86760-3-PBS)

    IF Staining of A431 using 86760-3-RR (same clone as 86760-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MINA antibody (86760-3-RR, Clone: 251692F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86760-3-PBS in a different storage buffer formulation.

MINA Recombinant monoclonal antibody, PBS Only

MINA Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86760-3-PBS

产品说明书

CloneNo. 251692F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

60S ribosomal protein L27a histidine hydroxylase, Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, EC:1.14.11.79, Histone lysine demethylase MINA, MDIG

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86760-3-PBS targets MINA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2838

Product name: Recombinant human MINA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 160-465 aa of BC014928

Sequence: SLVGSNVYITPAGSQGLPPHYDDVEVFILQLEGEKHWRLYHPTVPLAREYSVEAEERIGRPVHEFMLKPGDLLYFPRGTIHQADTPAGLAHSTHVTISTYQNNSWGDFLLDTISGLVFDTAKEDVELRTGIPRQLLLQVESTTVATRRLSGFLRTLADRLEGTKELLSSDMKKDFIMHRLPPYSAGDGAELSTPGGKLPRLDSVVRLQFKDHIVLTVLPDQDQSDETQEKMVYIYHSLKNSRETHMMGNEEETEFHGLRFPLSHLDALKQIWNSPAISVKDLKLTTDEEKESLVLSLWTECLIQVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MYC induced nuclear antigen
别名 60S ribosomal protein L27a histidine hydroxylase, Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, EC:1.14.11.79, Histone lysine demethylase MINA, MDIG
计算分子量 465 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC014928
基因名称 MINA
Gene ID (NCBI) 84864
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IUF8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)

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