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MINA Polyclonal antibody

MINA Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12214-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

MDIG, MINA, Mina53, MYC induced nuclear antigen, NO52, Nucleolar protein 52, Ribosomal oxygenase 2, RIOX2, ROX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, mouse liver tissue, A375 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12214-1-AP targets MINA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MINA fusion protein Ag2838 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MYC induced nuclear antigen
别名 MDIG, MINA, Mina53, MYC induced nuclear antigen, NO52, Nucleolar protein 52, Ribosomal oxygenase 2, RIOX2, ROX
计算分子量 465 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC014928
基因名称 MINA
Gene ID (NCBI) 84864
RRIDAB_2144191
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUF8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)

实验方案

Product Specific Protocols
WB protocol for MINA antibody 12214-1-APDownload protocol
IHC protocol for MINA antibody 12214-1-APDownload protocol
IP protocol for MINA antibody 12214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
  • KD Validated
mouseWB

EMBO J

NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.

Authors - Kashiwada Masaki M
humanWB

Cancer Res

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Authors - Lili Zhang
humanWB

Oncol Lett

Effects of mdig on proliferation and apoptosis of lung cancer cells.

Authors - Xiaohui Xu
  • KD Validated
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