验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, mouse liver tissue, A375 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12214-1-AP targets MINA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MINA fusion protein Ag2838 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MYC induced nuclear antigen |
| 别名 | MDIG, MINA, Mina53, MYC induced nuclear antigen, NO52, Nucleolar protein 52, Ribosomal oxygenase 2, RIOX2, ROX |
| 计算分子量 | 465 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC014928 |
| 基因名称 | MINA |
| Gene ID (NCBI) | 84864 |
| RRID | AB_2144191 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IUF8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MINA antibody 12214-1-AP | Download protocol |
| IHC protocol for MINA antibody 12214-1-AP | Download protocol |
| IP protocol for MINA antibody 12214-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
| ||
Cancer Res ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma. | ||
Oncol Lett Effects of mdig on proliferation and apoptosis of lung cancer cells.
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