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验证数据展示

  • WB analysis using 11630-1-AP (same clone as 11630-1-PBS)

    WB analysis using 11630-1-AP (same clone as 11630-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11630-1-AP (same clone as 11630-1-PBS)

    IHC staining of human colon cancer using 11630-1-AP (same clone as 11630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERRFI1/MIG6 antibody included
  • IF Staining of HepG2 using 11630-1-AP (same clone as 11630-1-PBS)

    IF Staining of HepG2 using 11630-1-AP (same clone as 11630-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MIG6; ERRFI1 antibody (11630-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MIG6; ERRFI1 Polyclonal antibody, PBS Only

MIG6; ERRFI1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11630-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ERRFI1/MIG6 Ready-To-Use IHC Kit. ERRFI1/MIG6 primary antibody included.

产品信息

11630-1-PBS targets MIG6; ERRFI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2202

Product name: Recombinant human MIG6; ERRFI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 113-462 aa of BC025337

Sequence: CGFKKLTVNGVCASTPPLTPIKNSPSLFPCAPLCERGSRPLPPLPISEALSLDDTDCEVEFLTSSDTDFLLEDSTLSDFKYDVPGRRSFRGCGQINYAYFDTPAVSAADLSYVSDQNGGVPDPNPPPPQTHRRLRRSHSGPAGSFNKPAIRISNCCIHRASPNSDEDKPEVPPRVPIPPRPVKPDYRRWSAEVTSSTYSDEDRPPKVPPREPLSPSNSRTPSPKSLPSYLNGVMPPTQSFAPDPKYVSSKALQRQNSEGSASKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEKFFREAEETNGGAQIQPLPADCGISSATEKPDSKTKMDLGGHVKRKHLSYVVSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ERBB receptor feedback inhibitor 1
别名
计算分子量 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC025337
基因名称 MIG6
Gene ID (NCBI) 54206
RRIDAB_2101525
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJM3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

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