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验证数据展示

  • WB analysis using 24288-1-AP

    WB analysis using 24288-1-AP

    mouse testis tissue was subjected to SDS PAGE followed by western blot with 24288-1-AP (MIF4GD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24288-1-AP

    IHC staining of mouse testis using 24288-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24288-1-AP (MIF4GD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MIF4GD Polyclonal antibody

MIF4GD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24288-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SLIP1, MIFD, MIF4G, hSLIP1, AD023

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24288-1-AP targets MIF4GD in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MIF4GD fusion protein Ag17931 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MIF4G domain containing
别名 SLIP1, MIFD, MIF4G, hSLIP1, AD023
计算分子量 256 aa, 29 kDa
观测分子量~25 kDa
GenBank蛋白编号BC033759
基因名称 MIF4GD
Gene ID (NCBI) 57409
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA9UHW6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIF4GD (MIF4G domain containing), also known as MIFD, AD023, or SLIP1, is a 222 amino acid protein that contains one MIF4G domain. Localized to both the nucleus and the cytoplasm, MIF4GD plays a role in the replication-dependent translation of histone mRNAs, which differ from most eukaryotic mRNAs in that they end with a stem-loop instead of a poly-A tail. Specifically, MIF4GD interacts with SLBP, eIF4G and DAP-5. Via its interaction with SLBP, MIF4GD is thought to be tethered to the stem loops of histone mRNAs where it may facilitate the circularizing of the mRNAs, thereby enhancing their translation. Depletion of MIF4GD results in reduced histone translation and may lead to cell death, suggesting that MIF4GD plays an important role in cell survival. Two isoforms of MIF4GD exist due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for MIF4GD antibody 24288-1-APDownload protocol
IHC protocol for MIF4GD antibody 24288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols