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MIF Polyclonal antibody

MIF Polyclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30957-1-AP

Print datasheet

Synonyms

GIF, Glif, Mif

验证数据展示

  • WB analysis using 30957-1-AP

    WB analysis using 30957-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30957-1-AP (MIF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 30957-1-AP

    IF Staining of Neuro-2a using 30957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of NIH/3T3 using 30957-1-AP

    FC experiment of NIH/3T3 using 30957-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Mouse MIF (30957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJ774A.1 cells, NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse brain tissue, mouse kidney tissue
Positive IF detected inNeuro-2a cells, NIH/3T3 cells
Positive FC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30957-1-AP targets MIF in WB, IF, FC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name macrophage migration inhibitory factor
Synonyms GIF, Glif, Mif
Calculated Molecular Weight13KD
Observed Molecular Weight13 kDa
GenBank Accession NumberNM_010798.2
Gene Symbol Mif
Gene ID (NCBI) 17319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

实验方案

Product Specific Protocols
WB protocol for MIF antibody 30957-1-APDownload protocol
IF protocol for MIF antibody 30957-1-APDownload protocol
FC protocol for MIF antibody 30957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols