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验证数据展示

  • WB analysis using 30957-1-AP

    WB analysis using 30957-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30957-1-AP (MIF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 30957-1-AP

    IF Staining of Neuro-2a using 30957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of NIH/3T3 using 30957-1-AP

    FC experiment of NIH/3T3 using 30957-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Mouse MIF (30957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MIF Polyclonal antibody

MIF Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 30957-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA and More (1)

别名

GIF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJ774A.1 cells, NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse brain tissue, mouse kidney tissue
Positive IF/ICC detected inNeuro-2a cells, NIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

30957-1-AP targets MIF in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用IF
经测试反应性 mouse, rat
文献引用反应性mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 macrophage migration inhibitory factor
别名 GIF
计算分子量13KD
观测分子量13 kDa
GenBank蛋白编号NM_010798.2
基因名称 Mif
Gene ID (NCBI) 17319
RRIDAB_3086428
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP34884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

实验方案

Product Specific Protocols
WB protocol for MIF antibody 30957-1-APDownload protocol
IF protocol for MIF antibody 30957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Chin Med

Salidroside protects against myocardial infarction via activating MIF-mediated mitochondrial quality control

Authors - Baiyang You
View Article