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验证数据展示

  • WB analysis using 17426-1-AP

    WB analysis using 17426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

MIER2 Polyclonal antibody

MIER2 Polyclonal Antibody for WB, ELISA
Cat No. 17426-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Mi-er2, Mesoderm induction early response protein 2, KIAA1193

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17426-1-AP targets MIER2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11491

Product name: Recombinant human MIER2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 197-545 aa of BC028203

Sequence: IMVGPQFQADLSNLHLNRHCEKIYENEDQLLWDPSVLPEREVEEFLYRAVKRRWHEMAGPQLPEGEAVKDSEQALYELVKCNFNVEEALRRLRFNVKVIRDGLCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFAQQTRLGRRKYVPSGTTDADQDLDGSDPDGPGRPRPEQDTLTGMRTDPLSVDGTAGGLDEPGVASDGLPSSEPGPCSFQQLDESPAVPLSHRPPALADPASYQPAVTAPEPDASPRLAVDFALPKELPLISSHVDLSGDPEETVAPAQVALSVTEFGLIGIGDVNPFLAAHPTCPAPGLHSEPLSHCNVMTC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mesoderm induction early response 1, family member 2
别名 Mi-er2, Mesoderm induction early response protein 2, KIAA1193
计算分子量 545 aa, 60 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC028203
基因名称 MIER2
Gene ID (NCBI) 54531
RRIDAB_2143931
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N344
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).

实验方案

Product Specific Protocols
WB protocol for MIER2 antibody 17426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols