MIDN Polyclonal antibody
MIDN Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
18939-1-AP targets MIDN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MIDN fusion protein Ag13492 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | midnolin |
| Synonyms | Midbrain nucleolar protein, MIDN, midnolin |
| Calculated Molecular Weight | 468 aa, 49 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC094778 |
| Gene Symbol | MIDN |
| Gene ID (NCBI) | 90007 |
| RRID | AB_2878569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q504T8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIDN antibody 18939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Biol CREB promotes beta cell gene expression by targeting Its coactivators to tissue-specific enhancers. | ||
Science The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation
| ||
Cancer Sci Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling |
