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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66384-1-Ig (same clone as 66384-1-PBS)

    WB analysis using 66384-1-Ig (same clone as 66384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

MICA Monoclonal antibody, PBS Only

MICA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66384-1-PBS

产品说明书

CloneNo. 1E2C8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

1E2C8, MIC A, MIC-A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66384-1-PBS targets MICA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3298

Product name: Recombinant human MICA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-383 aa of BC016929

Sequence: SWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETEEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQTFHVSAVAAAAIFVIIIFYVRCCKKKTSAAEGPELVSLQVLDQHPVGTSDHRDATQLGFQPLMSDLGSTGSTEGT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 MHC class I polypeptide-related sequence A
别名 1E2C8, MIC A, MIC-A
计算分子量 383 aa, 43 kDa
观测分子量 43-50 kDa
GenBank蛋白编号BC016929
基因名称 MICA
Gene ID (NCBI) 100507436
RRIDAB_2881760
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ29983
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).