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验证数据展示

  • WB analysis using 83718-5-RR (same clone as 83718-5-PBS)

    WB analysis using 83718-5-RR (same clone as 83718-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83718-5-RR (MIB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83718-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83718-5-RR (same clone as 83718-5-PBS)

    FC experiment of HeLa using 83718-5-RR (same clone as 83718-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83718-5-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83718-5-RR (same clone as 83718-5-PBS)

    FC experiment of SH-SY5Y using 83718-5-RR (same clone as 83718-5-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83718-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83718-5-RR

    Affinity and Kinetic Characterization of 83718-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83718-5-RR against Human MIB1 were performed. The affinity constant is 5.15 nM.

MIB1 Recombinant monoclonal antibody, PBS Only

MIB1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83718-5-PBS

产品说明书

CloneNo. 240676E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

240676E4, DAPK interacting protein 1, DAPK-interacting protein 1, DIP 1, DIP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83718-5-PBS targets MIB1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2507

Product name: Recombinant human MIB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 707-1006 aa of BC022403

Sequence: LRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCANLMKKCVQCRAVVERRVPFIMCCGGKSSEDATDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mindbomb homolog 1 (Drosophila)
别名 240676E4, DAPK interacting protein 1, DAPK-interacting protein 1, DIP 1, DIP1
计算分子量 110 kDa
观测分子量 110 kDa
GenBank蛋白编号BC022403
基因名称 MIB1
Gene ID (NCBI) 57534
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ86YT6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

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