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MGST1 Polyclonal antibody

MGST1 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 15906-1-AP

Print datasheet

Synonyms

GST12, MGST, MGST I, MGST1, Microsomal GST 1, Microsomal GST I

验证数据展示

  • WB analysis using 15906-1-AP

    WB analysis using 15906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15906-1-AP (MGST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15906-1-AP

    IHC staining of human liver using 15906-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 15906-1-AP

    IHC staining of human liver using 15906-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15906-1-AP

    IF Staining of MCF-7 using 15906-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGST1 antibody (15906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue，rat liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15906-1-AP targets MGST1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Tested Reactivity human, mouse, rat
Immunogen MGST1 fusion protein Ag8498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microsomal glutathione S-transferase 1
Synonyms GST12, MGST, MGST I, MGST1, Microsomal GST 1, Microsomal GST I
Calculated Molecular Weight 155 aa, 18 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC005923
Gene Symbol MGST1
Gene ID (NCBI) 4257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Microsomal glutathione S-transferase 1 (MGST1) is a membrane-bound transferase involved in exogenous detoxification and protection of cells from oxidative stress. MGST1 is mainly localized in the mitochondria, the plasma membrane of the endoplasmic reticulum (ER). The active form of MGST1 is a noncovalently associated homotrimer that binds one substrate glutathione molecule/trimer (PMID: 14726533; PMID: 11101503). The theoretical molecular weight of MGST1 is 17 kDa, and its homomeric molecular weight is 51-53 kDa.

实验方案

Product Specific Protocols
WB protocol for MGST1 antibody 15906-1-APDownload protocol
IHC protocol for MGST1 antibody 15906-1-APDownload protocol
IF protocol for MGST1 antibody 15906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols