验证数据展示
经过测试的应用
| Positive IHC detected in | human colon cancer tissue, human small intestine tissue, human breast cancer tissue, human lymphoma tissue, human breast hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10734-1-AP targets MGP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MGP fusion protein Ag1091 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | matrix Gla protein |
| 别名 | GIG36, MGLAP, matrix Gla protein, Cell growth-inhibiting gene 36 protein |
| 计算分子量 | 103 aa, 13 kDa |
| GenBank蛋白编号 | BC005272 |
| 基因名称 | MGP |
| Gene ID (NCBI) | 4256 |
| RRID | AB_2297660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08493 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Matrix Gla protein (MGP), a vitamin K-dependent protein, is recognized as a calcification inhibitor in vascular tissue. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MGP antibody 10734-1-AP | Download protocol |
| IF protocol for MGP antibody 10734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Circulation Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis. | ||
Nat Commun Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease. | ||
Arthritis Rheum Juvenile dermatomyositis calcifications selectively displayed markers of bone formation. | ||
Aging (Albany NY) Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1. | ||
FASEB J 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes injury-induced vascular neointima formation in mice. | ||
Int J Obes (Lond) Using gene expression to predict the secretome of differentiating human preadipocytes. |
