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验证数据展示

  • IHC staining of human liver cancer using 14986-1-PBS

    IHC staining of human liver cancer using 14986-1-PBS

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MGLL antibody included
  • IHC staining of human liver cancer using 14986-1-PBS

    IHC staining of human liver cancer using 14986-1-PBS

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MGLL antibody included
  • WB analysis of HepG2 using 14986-1-AP (same clone as 14986-1-PBS)

    WB analysis of HepG2 using 14986-1-AP (same clone as 14986-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 14986-1-AP (same clone as 14986-1-PBS)

    IP experiment of mouse liver using 14986-1-AP (same clone as 14986-1-PBS)

    IP result of anti-MGLL (IP:14986-1-AP, 4ug; Detection:14986-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • WB analysis using 14986-1-AP (same clone as 14986-1-PBS)

    WB analysis using 14986-1-AP (same clone as 14986-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 14986-1-AP (same clone as 14986-1-PBS)

    IHC staining of human liver using 14986-1-AP (same clone as 14986-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MGLL antibody included
  • IHC staining of human kidney using 14986-1-AP (same clone as 14986-1-PBS)

    IHC staining of human kidney using 14986-1-AP (same clone as 14986-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MGLL antibody included
  • Featured Product
  • KD/KO Validated

MGLL Polyclonal antibody, PBS Only

MGLL Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14986-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MGLL Ready-To-Use IHC Kit. MGLL primary antibody included.

产品信息

14986-1-PBS targets MGLL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6970

Product name: Recombinant human MGLL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC000551

Sequence: METGPEDPSSMPEESSPRRTPQSIPYQDLPHLVNADGQYLFCRYWKPTGTPKALIFVSHGAGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 monoglyceride lipase
别名 EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase
计算分子量 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC000551
基因名称 MGLL
Gene ID (NCBI) 11343
RRIDAB_2143189
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA0A0C4DFN3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

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