验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human gliomas tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11454-1-AP targets MZB1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2015 Product name: Recombinant human MZB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC021275 Sequence: MRLSLPLLLLLLGAWAIPGGLGDRAPLTATAPQLDDEEMYSAHMPAHLRCDACRAVAYQMWQNLAKAETKLHTSNSGGRRELSELVYTDVLDRSCSRNWQDYGVREVDQVKRLTGPGLSEGPEPSISVMVTGGPWPTRLSRTCLHYLGEFGEDQIYEAHQQGRGALEALLCGGPQGACSEKVSATREEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hypothetical protein MGC29506 |
| 别名 | hypothetical protein MGC29506, MZB1, PACAP, pERp1 |
| 计算分子量 | 189 aa, 21 kDa |
| 观测分子量 | 19-21 kDa |
| GenBank蛋白编号 | BC021275 |
| 基因名称 | MZB1 |
| Gene ID (NCBI) | 51237 |
| RRID | AB_832820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WU39 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MZB1 antibody 11454-1-AP | Download protocol |
| WB protocol for MZB1 antibody 11454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor suppressor in hepatocellular carcinoma. | ||
J Pathol Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney. | ||
Int J Cancer Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer. | ||
J Biol Chem Transgenic expression of microRNA-185 causes a developmental arrest of T cells by targeting multiple genes including Mzb1. | ||
Arthritis Res Ther Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes. | ||
Oncol Lett MZB1 expression indicates poor prognosis in estrogen receptor-positive breast cancer. |
