• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MGAT4A Polyclonal antibody

MGAT4A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25109-1-AP

Print datasheet

Synonyms

GlcNAc T IVa, GNT IV, GNT IVA, MGAT4A

验证数据展示

  • WB analysis of HeLa using 25109-1-AP

    WB analysis of HeLa using 25109-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25109-1-AP (MGAT4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25109-1-AP

    IHC staining of human small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25109-1-AP (MGAT4A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 25109-1-AP

    IHC staining of human small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25109-1-AP (MGAT4A Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25109-1-AP is MGAT4A Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MGAT4A fusion protein Ag18956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Synonyms GlcNAc T IVa, GNT IV, GNT IVA, MGAT4A
Calculated Molecular Weight 535 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC127107
Gene Symbol MGAT4A
Gene ID (NCBI) 11320
RRIDAB_2879901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MGAT4A antibody 25109-1-APDownload protocol
IHC protocol for MGAT4A antibody 25109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Biochim Biophys Acta Mol Cell Res

N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility

Authors - Depeng Yang
View Article