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  • Featured Product
  • KD/KO Validated

MGAT3 Polyclonal antibody

MGAT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17869-1-AP

Print datasheet

Synonyms

GGNT3, GlcNAc T III, GNT III, GNT3, MGAT3

验证数据展示

  • WB analysis of COLO 320 using 17869-1-AP

    WB analysis of COLO 320 using 17869-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17869-1-AP

    IHC staining of human colon cancer using 17869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 17869-1-AP

    IHC staining of human colon cancer using 17869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, Caco-2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17869-1-AP targets MGAT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MGAT3 fusion protein Ag12352 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
Synonyms GGNT3, GlcNAc T III, GNT III, GNT3, MGAT3
Calculated Molecular Weight 533 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC075026
Gene Symbol MGAT3
Gene ID (NCBI) 4248
RRIDAB_10597697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.

实验方案

Product Specific Protocols
WB protocol for MGAT3 antibody 17869-1-APDownload protocol
IHC protocol for MGAT3 antibody 17869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Theranostics

MGAT3-mediated glycosylation of tetraspanin CD82 at asparagine 157 suppresses ovarian cancer metastasis by inhibiting the integrin signaling pathway.

Authors - Jun Li
  • KD Validated
View Article

Neuropharmacology

DHEC mesylate attenuates pathologies and aberrant bisecting N-glycosylation in Alzheimer's disease models

Authors - Yue Wang
View Article