验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human placenta tissue, human testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25498-1-AP targets MFSD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22232 Product name: Recombinant human MFSD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 453-530 aa of BC092414 Sequence: AGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDEASSSGCSETDSTELASIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major facilitator superfamily domain containing 2 |
| 别名 | FP1147, HMFN0656, MFSD2, MFSD2A, PP10484, PP9177, RP3 342P20.1, UNQ300/PRO341 |
| 计算分子量 | 543 aa, 60 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC092414 |
| 基因名称 | MFSD2 |
| Gene ID (NCBI) | 84879 |
| RRID | AB_2880103 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NA29 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MFSD2, also named as MFSD2A, belongs to a large family of presumptive carbohydrate transporters with 10-12 membrane-spanning domains, is located at chromosomal position 1p34.2, and is conserved in evolution. It plays a role in thermogenesis via beta-adrenergic signaling pathway. It may be the main plasma membrane tunicamycin transporter. MFSD2 has three isoforms with MW 50 kDa and 59-60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MFSD2 antibody 25498-1-AP | Download protocol |
| IHC protocol for MFSD2 antibody 25498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuro Oncol Wnt signaling regulates MFSD2A-dependent drug delivery through endothelial transcytosis in glioma | ||
bioRxiv Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM | ||
Sci Adv Modulation of blood-tumor barrier transcriptional programs improves intratumoral drug delivery and potentiates chemotherapy in GBM |
