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验证数据展示

  • IHC staining of human placenta using 25498-1-AP

    IHC staining of human placenta using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 25498-1-AP

    IHC staining of human placenta using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 25498-1-AP

    WB analysis of HepG2 using 25498-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25498-1-AP (MFSD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MFSD2 Polyclonal antibody

MFSD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25498-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

FP1147, HMFN0656, MFSD2, MFSD2A, PP10484, PP9177, RP3 342P20.1, UNQ300/PRO341

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25498-1-AP targets MFSD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 MFSD2 fusion protein Ag22232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major facilitator superfamily domain containing 2
别名 FP1147, HMFN0656, MFSD2, MFSD2A, PP10484, PP9177, RP3 342P20.1, UNQ300/PRO341
计算分子量 543 aa, 60 kDa
观测分子量 62 kDa
GenBank蛋白编号BC092414
基因名称 MFSD2
Gene ID (NCBI) 84879
RRIDAB_2880103
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NA29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFSD2, also named as MFSD2A, belongs to a large family of presumptive carbohydrate transporters with 10-12 membrane-spanning domains, is located at chromosomal position 1p34.2, and is conserved in evolution. It plays a role in thermogenesis via beta-adrenergic signaling pathway. It may be the main plasma membrane tunicamycin transporter. MFSD2 has three isoforms with MW 50 kDa and 59-60 kDa.

实验方案

Product Specific Protocols
WB protocol for MFSD2 antibody 25498-1-APDownload protocol
IHC protocol for MFSD2 antibody 25498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Neuro Oncol

Wnt signaling regulates MFSD2A-dependent drug delivery through endothelial transcytosis in glioma

Authors - Yuan Xie
View Article
humanWB

bioRxiv

Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM

Authors - Jorge L Jimenez-Macias
View Article