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验证数据展示

  • WB analysis using 87246-1-RR (same clone as 87246-1-PBS)

    WB analysis using 87246-1-RR (same clone as 87246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87246-1-RR (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87246-1-PBS in a different storage buffer formulation.

  • IP experiment of T-47D using 87246-1-RR (same clone as 87246-1-PBS)

    IP experiment of T-47D using 87246-1-RR (same clone as 87246-1-PBS)

    IP result of anti-MFN1 (IP:87246-1-RR, 4ug; Detection:87246-1-RR 1:1000) with T-47D cells lysate 1120 ug. This data was developed using the same antibody clone with 87246-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 87246-1-RR (same clone as 87246-1-PBS)

    IHC staining of mouse liver using 87246-1-RR (same clone as 87246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 87246-1-RR (MFN1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87246-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN1 antibody included
  • Affinity of 87246-1-RR

    Affinity of 87246-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87246-1-RR against Human MFN1 were performed. The affinity constant is below 1 pM.

MFN1 Recombinant monoclonal antibody, PBS Only

MFN1 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 87246-1-PBS

产品说明书

CloneNo. 250228A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

EC:3.6.5.-, Fzo homolog, hfzo1, hfzo2, mitofusin 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MFN1 Ready-To-Use IHC Kit. MFN1 primary antibody included.

产品信息

87246-1-PBS targets MFN1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4762

Product name: Recombinant human MFN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-351 aa of BC040557

Sequence: EFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitofusin 1
别名 EC:3.6.5.-, Fzo homolog, hfzo1, hfzo2, mitofusin 1
计算分子量 741 aa, 84 kDa
观测分子量84 kDa
GenBank蛋白编号BC040557
基因名称 MFN1
Gene ID (NCBI) 55669
RRIDAB_3745427
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWA4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in the outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

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