验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, mouse heart tissue, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human lung tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17661-1-AP targets MFAP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag11920 Product name: Recombinant human MFAP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC062415 Sequence: MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGVGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microfibrillar-associated protein 4 |
| 别名 | Microfibril-associated glycoprotein 4 |
| 计算分子量 | 255 aa, 29 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC062415 |
| 基因名称 | MFAP4 |
| Gene ID (NCBI) | 4239 |
| RRID | AB_2142355 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55083 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MFAP4 antibody 17661-1-AP | Download protocol |
| WB protocol for MFAP4 antibody 17661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Thorax A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD. | ||
Front Pharmacol MFAP4 is a novel prognostic biomarker in glioma correlating with immunotherapy resistance and ferroptosis | ||
J Proteome Res Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples. | ||
J Proteomics FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution | ||
BMC Biol The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis |
