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验证数据展示

  • WB analysis of C2C12 using 30185-1-AP

    WB analysis of C2C12 using 30185-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 30185-1-AP (MEX3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of SGC-7901 using 30185-1-AP

    WB analysis of SGC-7901 using 30185-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30185-1-AP (MEX3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

MEX3B Polyclonal antibody

MEX3B Polyclonal Antibody for WB, ELISA
Cat No. 30185-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

MEX 3B, RING finger and KH domain-containing protein 3, RKHD3, RNA-binding protein MEX3B, RNF195

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inC2C12 cells, SGC-7901 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30185-1-AP targets MEX3B in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 MEX3B fusion protein Ag32899 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mex-3 homolog B (C. elegans)
别名 MEX 3B, RING finger and KH domain-containing protein 3, RKHD3, RNA-binding protein MEX3B, RNF195
计算分子量 569 aa, 59 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC036211
基因名称 MEX3B
Gene ID (NCBI) 84206
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZN04
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human MEX3B (hMEX3B) protein is an RNA-binding protein that contains two KH domains at the N-terminus and a RING domain at its C-terminus, which has the activity of E3 ubiquitin ligase and is essential for RNA degradation.

实验方案

Product Specific Protocols
WB protocol for MEX3B antibody 30185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols