验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, SGC-7901 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30185-1-AP targets MEX3B in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag32899 Product name: Recombinant human MEX3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-458 aa of BC036211 Sequence: YFGGGTSSSAAATQRLADYSPPSPALSFAHNGNNNNNGNGYTYTAGGEASVPSPDGCPELQPTFDPAPAPPPGAPLIWAQFERSPGGGPAAPVSSSCSSSASSSASSSSVVFPGSGASAPSNANLGLLVHRRLHPGTSCPRLSPPLHMAPGAGEHHLAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mex-3 homolog B (C. elegans) |
| 别名 | MEX 3B, RING finger and KH domain-containing protein 3, RKHD3, RNA-binding protein MEX3B, RNF195 |
| 计算分子量 | 569 aa, 59 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC036211 |
| 基因名称 | MEX3B |
| Gene ID (NCBI) | 84206 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZN04 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human MEX3B (hMEX3B) protein is an RNA-binding protein that contains two KH domains at the N-terminus and a RING domain at its C-terminus, which has the activity of E3 ubiquitin ligase and is essential for RNA degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEX3B antibody 30185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
