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MEX3A Polyclonal antibody

MEX3A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30865-1-AP

Print datasheet

Synonyms

mex 3 homolog A (C. elegans), MEX 3A, MEX3A, RKHD4, RNA binding protein MEX3A

验证数据展示

  • WB analysis using 30865-1-AP

    WB analysis using 30865-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30865-1-AP (MEX3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, SKOV-3 cells, HCT 116 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30865-1-AP targets MEX3A in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen MEX3A fusion protein Ag33619 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mex-3 homolog A (C. elegans)
Synonyms mex 3 homolog A (C. elegans), MEX 3A, MEX3A, RKHD4, RNA binding protein MEX3A
Calculated Molecular Weight54 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberNM_001093725
Gene Symbol MEX3A
Gene ID (NCBI) 92312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA1L020
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEX3A is a member of the MEX3 family, which widely exists in mammalian cells and encodes four different genes, MEX3A, MEX3B, MEX3C, and MEX3D. The MEX3 protein of mammals is composed of two RNA-binding KH domains and a ring domain with E3 ubiquitin ligase activity at the C terminal. The ring finger domain endows human MEX3 protein the ability to regulate ubiquitination of target proteins and then influencing their protein stability and subcellular localization.MEX3A plays complex and diverse roles in the development of various cancers. MEX3A plays a pivotal role in the post-transcriptional procession of mRNA and gene expression. MEX3A gene expression may be closely connected with malignant tumor development and progression. In most literature, MEX3A detects molecular weights between 60 and 70kDa (PMID:33159833,37845346,36354374,35490173,34976441,33159833)

实验方案

Product Specific Protocols
WB protocol for MEX3A antibody 30865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols